fig9

Figure 9. Effects of EVs functionalized with p17-derived peptides on angiogenesis in HUVECs. Angiogenic activity evaluated by tube formation assay in HUVECs treated with nEVs, EV-CD63-GFP, or EVs functionalized with the indicated p17-derived peptides. Images represent three independent experiments with similar results (original magnification 10×). Scale bar: 200 µm. Data are expressed as mean ± SD of three independent experiments performed in triplicate. Statistical analysis was performed by one-way ANOVA, followed by Bonferroni’s post hoc test (^****P < 0.0001). ANOVA: Analysis of variance; CD63: cluster of differentiation 63; EV: extracellular vesicle; GFP: green fluorescent protein; HUVEC: human umbilical vein endothelial cell; nEV: native extracellular vesicle; SD: standard deviation.