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Figure 5. Effects of the p17-derived peptide F3 39-43 on pro-migratory and angiogenic activity of HUVECs under stress and standard conditions. (A and C) Pro-migratory activity evaluated by wound healing assay in HUVECs cultured under serum-starved conditions (A) and under standard conditions (C), treated with NT, SCR, F3, or F3 39-43; (B and D) angiogenic activity was evaluated by tube formation assay in HUVECs cultured under serum-starved conditions (B) and under standard conditions (D), treated as indicated. Scale bar: 200 µm. (A-D) Images are representative of three independent experiments with similar results (original magnification, ×10). Data are expressed as mean ± SD of three independent experiments performed in triplicate. Statistical analysis was performed by one-way ANOVA followed by Bonferroni’s post hoc test (^****P < 0.0001). ANOVA: Analysis of variance; HUVEC: human umbilical vein endothelial cell; NT: no treatment; SCR: scrambled peptide; SD: standard deviation.