fig4

Figure 4. Effects of p17 F3-derived peptides on migratory and tube formation activity of HUVECs under standard conditions. (A) Pro-migratory activity evaluated by wound healing assay in HUVECs cultured under standard conditions, treated with NT, SCR, full-length p17, or the indicated F3-derived peptides (F3; F3 37-52; F3 37-44; F3 40-44); (B) angiogenic activity evaluated by tube formation assay in HUVECs cultured under standard conditions, treated as indicated. Scale bar: 200 µm. (A and B) Images are representative of three independent experiments with similar results (original magnification, ×10). Data are expressed as mean ± SD of three independent experiments performed in triplicate. Statistical analysis was performed by one-way ANOVA, followed by Bonferroni’s post hoc test (^****P < 0.0001). ANOVA: Analysis of variance; HUVEC: human umbilical vein endothelial cell; NT: no treatment; SCR: scrambled peptide; SD: standard deviation.