fig7

Figure 7. Percentage of eight phospholipid classes in SSL or IF mitochondria, SSL- or IF-associated heart EVs (SHEV, IHEV, respectively), or left ventricular tissue. Graphs show the percentage of the sum of detected species for (A) PA, (B) PC, (C) PE, (D) PG, (E) PI, (F) PS, (G) SM, and (H) CL. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001; N = 2 donor hearts. Because of the limited biological sample size (N = 2), these data are presented as exploratory/descriptive findings. Individual donor values are shown. Error bars show SEM. Analysis was performed using ordinary one-way ANOVA with multiple comparisons corrected using Tukey’s test. Comparisons in this dataset were between SSL Mito and SHEV, IF Mito and IHEV, SHEV and Tissue, and IHEV and Tissue. SSL: Subsarcolemmal; IF: interfibrillar; EV: extracellular vesicle; SHEV: subsarcolemmal heart extracellular vesicle; IHEV: interfibrillar heart extracellular vesicle; PA: phosphatidic acid; PC: phosphatidylcholine; PE: phosphatidylethanolamine; PG: phosphatidylglycerol; PI: phosphatidylinositol; PS: phosphatidylserine; SM: sphingomyelin; CL: cardiolipin; SEM: standard error of the mean; ANOVA: analysis of variance.