fig4

Figure 4. PPI network and functional analysis of OEC-sEVs. (A) Expression levels of phagocytosis-related proteins (Pros1, Rab5b, Gas6, MerTK, Rab7a, Rab5a) and Capzb in OEC-sEV groups vs. OEC groups; (B) PPI network analysis of the phagocytosis pathway-related DEPs and MerTK; (C) GO (Biological Process) analysis of highly expressed proteins related to phagocytosis in the OEC-sEV group; (D) GO (Molecular Function) analysis of highly expressed proteins related to phagocytosis in the OEC-sEV group; (E) GO (Cellular Component) analysis of highly expressed proteins related to phagocytosis in the OEC-sEV group; (F) KEGG pathway enrichment for highly expressed proteins related to phagocytosis in the OEC-sEV group. n = 3 biologically independent OEC cultures and n = 3 biologically independent sEV preparations. Red boxes highlight the critical pathways associated with RPE phagocytosis. Capzb: Capping actin protein of muscle Z-line subunit beta; DEPs: differentially expressed proteins; Gas6: growth arrest-specific protein 6; GO: Gene Ontology; KEGG: Kyoto Encyclopedia of Genes and Genomes; MerTK: MER proto-oncogene, tyrosine kinase; OEC: olfactory ensheathing cell; OEC-sEVs: olfactory ensheathing cell-derived small extracellular vesicles; PPI: protein-protein interaction; Pros1: protein S1; Rab5a: Ras-related protein Rab-5A; Rab5b: Ras-related protein Rab-5B; Rab7a: Ras-related protein Rab-7A; RPE: retinal pigment epithelium; sEV: small extracellular vesicle; sEVs: small extracellular vesicles.