fig6

Figure 6. Comparative GO and KEGG pathway enrichment analysis of lEVs and sEVs derived from MEG-01 cells. (A-C) Comparative GO analysis of proteins identified in lEVs and sEVs isolated from MEG-01 cells. GO terms are grouped into the three main annotation categories: cellular component (A), molecular function (B), and biological process (C); (D) KEGG pathway enrichment analysis of proteins associated with lEVs (left) and sEVs (right) derived from MEG-01 cells. Enriched pathways are ranked according to fold enrichment, highlighting both shared and vesicle-type-specific pathways. For both GO and KEGG analyses, only proteins detected in all three biological replicates of each EV subtype were included. EV: Extracellular vesicle; GO: Gene Ontology; KEGG: Kyoto Encyclopedia of Genes and Genomes; lEV: large extracellular vesicle; sEV: small extracellular vesicle.