fig5

Figure 5. Proteomic comparison of DG-purified lEVs and sEVs from MEG-01 cells. (A) Venn diagram showing the overlap of proteins identified in lEVs, sEVs, and the top 100 proteins listed in ExoCarta; (B) PCA of proteomic profiles reveals clear separation of proteomes between lEVs and sEVs from MEG-01 cells. Each shape represents a biological replicate (n = 3); (C) Heatmap displaying the relative abundance of proteins detected in lEVs and sEVs; (D) Volcano plot comparing protein abundance between lEVs and sEVs. All proteomic analyses were performed using EVs isolated in three independent experiments for each group. PCA (B), heatmap (C), and volcano plot (D) were generated based on these biological replicates. DG: Density gradient; EV: extracellular vesicle; lEV: large extracellular vesicle; PCA: principal component analysis; sEV: small extracellular vesicle.