fig5

Figure 5. Proteomic and multi-omic characterization of PROSPR-enriched EVs from blood and plasma. (A) Venn diagram showing the number of proteins identified in blood and plasma-derived EVs; (B) Overlap of identified proteins with the top 100 most common EV-associated proteins listed in the ExoCarta database; (C) Overlap of identified proteins with the top 100 proteins in the Vesiclepedia database; (D) Functional enrichment analysis of biological processes associated with proteins exclusively identified in blood-derived EVs. The list of proteins involved in each pathway is listed in Supplementary Table 9; (E) Equivalent functional enrichment analysis for proteins exclusively detected in plasma-derived EVs. Dot size represents the number of proteins per functional category, and color indicates the adjusted P-value for enrichment. The list of proteins involved in each pathway is listed in Supplementary Table 10; (F) Multi-omic integration of proteomic and lipidomic profiles of blood-derived EVs. The network shows correlations between membrane-associated proteins and lipid species exclusively present in blood-derived EVs. Pearson correlations (r > 0.5) are shown in red, and negative correlations (r < -0.5) in blue. Analyses were performed using independent biological samples. PROSPR: PRotein Organic Solvent PRecipitation; EVs: extracellular vesicles; RHAG: Rh-associated glycoprotein; RAC1: Rac Family Small GTPase 1; CALM1: calmodulin 1; ANXA5: annexin A5; ANK1: ankyrin 1; CDC42: cell division cycle 42; DCD: dermcidin; FABP5: fatty acid binding protein 5; CLIC1: chloride intracellular channel protein 1; ANXA2: annexin A2; MKD: megakaryocyte differentiation; RT: retention Time; LysoPE: lysophosphatidylethanolamine; CerP: ceramide Phosphate; Chol: cholesterol; MAG: monoacylglycerol; CE: cholesteryl ester; DAG: diacylglycerol; LysoPC: lysophosphatidylcholine; FA: fatty acid; PC: phosphatidylcholine; SM: sphingomyelin; PE: phosphatidylethanolamine; Cer: ceramides; PS: phosphatidylserine; TG: triradylglycerols.