fig1

Figure 1. Preparation and characterization of ADSC-EV. (A) Morphology of primary ADSCs at passage 4 (P4) at 80% confluence (scale bar: 100 µm); (B) Schematic workflow for isolating ADSC-EV from the ADSC culture supernatant; (C) Representative TEM image of isolated ADSC-EV (scale bar: 100 nm); (D) NTA of ADSC-EV; (E) Immunoblots for typical EV markers (CD9, CD81, TSG101, Alix) and negative marker (Calnexin) in isolated ADSC-EV and conditional CM; (F) NanoFCM of isolated ADSC-EV for positive markers CD9, CD81, and CD63. ADSC-EV: Adipose-derived stem cell extracellular vesicle; TEM: transmission electron microscopy; NTA: nanoparticle tracking analysis; CM: culture medium; NanoFCM: nano-flow cytometry analysis.