fig8

Figure 8. The impact of TC-derived sEVs on TC development in vitro and in vivo. The effects of TC-derived sEVs on TC cell proliferation determined by CCK-8 assays (A and B) or colony formation assays (C and D); Representative images of migration assays (E and F) and invasion assays (G and H) of TC cells subjected to PBS or plasma sEVs from TC patients; (I) Schematic diagram of mouse experiments; (J) TPC-1 tumors were photographed when the tumor-bearing mice were sacrificed; (K) The tumor volume was compared between two groups. The data was analyzed using the Student’s t-test. ^*P value < 0.05; ^**P value < 0.01; ^***P value < 0.001; ns: not significant. TC: Thyroid carcinoma; sEVs: small extracellular vesicles; CCK-8: cell counting kit-8; PBS: phosphate-buffered saline; OD450: optical density at 450 nm.