fig8
From: Comparison of methods for isolation of extracellular vesicles from bronchoalveolar lavage fluid
Figure 8. Measurement of RNA and dsDNA in nucleic acids isolated from extracellular vesicle preparations. EV nucleic acid content for RNA and dsDNA was measured using InvitrogenTM Quant-iTTM RNA Assay and InvitrogenTM Quant-iTTM PicoGreenTM dsDNA Assay Kits. (A) Comparison of RNA and dsDNA in EV preparations using SEC, UC, and EE; (B) Comparison of RNA and dsDNA found in the EV and non-EV fractions obtained by size exclusion chromatography. n = 2 subjects run in triplicate. EV: Extracellular vesicle; RNA: ribonucleic acid; dsDNA: double-stranded deoxyribonucleic acid; SEC: size exclusion chromatography; UC: ultracentrifugation; EE: exoEasy.
