fig5
From: Comparison of methods for isolation of extracellular vesicles from bronchoalveolar lavage fluid
Figure 5. Total protein and nucleic acid in EV and non-EV fractions collected from SEC as determined by BCA assay and NanoDrop. (A) EVs isolated from acellular BAL contain significantly less protein than the non-EV fraction (P = 0.0009); (B) EVs isolated from acellular BAL contain significantly more nucleic acid material than the non-EV fraction (P < 0.0001). n = 40 subjects. Analysis performed using unpaired two-tailed t-test assuming equal variance. EVs: Extracellular vesicles; SEC: size exclusion chromatography; BAL: bronchoalveolar lavage; BCA: bicinchoninic acid.
