fig4

From: Special delivery - extracellular vesicles released by commensal gut bacteria deliver bioactive protein to distal organs

Figure 4. BEV distribution in vivo following colonisation with NanoLuc-BEV-producing B. thetaiotaomicron. (A) GF or SPF mice were monocolonised for 7 days, after which individual organs were excised and imaged using the Bruker In vivo Xtreme system. Substrate was injected intraperitoneally 5 min prior to euthanasia and organ excision. Representative images from GF (n = 6) or SPF (n = 7) mice are shown; (B) Quantification of luminescence from each organ. Error bars represent mean ± SD. Red dotted line indicates LOD. ns: Not significant; BEV: bacterially derived extracellular vesicle; NanoLuc: Nanoluciferase; B. thetaiotaomicron: Bacteroides thetaiotaomicron; GF: germ-free; SPF: specific pathogen-free; MLN: mesenteric lymph node; SD: standard deviation; ns: not significant; LOD: limit of detection.