fig7

Figure 7. hsa-let-7g-5p as a key component of UCMSC-NVs regulates inflammation by targeting the NF-κB pathway. (A) Top 20 miRNAs identified in UCMSC-NVs; (B) RT-qPCR analysis of hsa-let-7g-5p expression in murine lung tissue. n = 5 per group. Data represent means ± SD. Statistical analysis was performed by one-way ANOVA. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ns: not significant; (C) Multiple species alignment illustrating the high sequence conservation of hsa-let-7g-5p across species; (D and E) GO and KEGG enrichment analyses of miRNAs. UCMSC-NVs: Nanovesicles originating from human umbilical cord mesenchymal stem cells; NF-κB: nuclear factor κB; miRNAs: microRNAs; RT-qPCR: reverse transcription quantitative polymerase chain reaction; SD: standard deviation; ANOVA: analysis of variance; GO: Gene Ontology; KEGG: Kyoto Encyclopedia of Genes and Genomes; LPS: lipopolysaccharide.