fig1

Figure 1. Characterization of MSC-Exo and their effects on LPS-induced endothelial migration dysfunction and apoptosis. (A) Effects of MSC-Exo collected under different conditions on LPS-induced migration dysfunction in PMVECs. Quantitative analysis of migrated cells. Scale bar = 50 μm. The experiment was independently repeated three times; (B) Protective effects of MSC-Exo collected under different conditions on LPS-induced apoptosis in PMVECs. Quantitative analysis of apoptosis rate; (C) Western blot detection of Bcl-2 and Bax protein expression in PMVECs treated with MSC-Exo. The experiment was independently repeated three times. Data are presented as mean ± SD. Statistical analysis was performed using one-way ANOVA followed by Tukey’s multiple comparisons test. ^*P < 0.05; ^**P < 0.01; ^***P < 0.001. MSC: Mesenchymal stem cell; MSC-Exo: mesenchymal stem cell-derived exosomes; Exo: exosome; LPS: lipopolysaccharide; PMVECs: pulmonary microvascular endothelial cells; NC: normal control; Exo (MSCs): exosomes derived from mesenchymal stem cells; Exo (LPS + MSCs): exosomes derived from lipopolysaccharide-treated mesenchymal stem cells; Bcl-2: B-cell lymphoma 2; Bax: Bcl-2-associated X protein; SD: standard deviation; ANOVA: analysis of variance.