fig3

Figure 3. Effects of exosomal LYPLAL1-DT on angiogenesis of HUVEC. (A and B) H446-derived exosomes can be internalized by HUVEC (Scale bar: 50 and 10 μm); (C) The expression of LYPLAL1-DT in HUVECs was significantly higher following treatment with H446 OE-Exosomes compared to H446 OC-Exosomes; (D) The proliferation ability of HUVECs was significantly enhanced by H446 OE-Exosome treatment; (E) The migration ability of HUVECs was significantly increased by H446 OE-Exosome treatment; (F) The tube formation ability (tube length and branch point) of HUVECs was significantly promoted by H446 OE-Exosome treatment (scale bar: 100 μm). Data are presented as mean ± SD. Differences between the two groups were analyzed by Student’s t-test, while comparisons among more than two groups were analyzed by one-way ANOVA. (^*P < 0.05; ^**P < 0.01; ^***P < 0.001; ^****P < 0.0001). LYPLAL1-DT: LYPLAL1 divergent transcript; HUVEC: human umbilical vein endothelial cell; OE-Exo: exosomes from overexpressing cells; OC-Exo: exosomes from control cells; SD: standard deviation; ANOVA: analysis of variance; PKH67: PKH67 green fluorescent cell linker.