fig1

Figure 1. Detection of plasmids YEp352 and pRS316 by PCR amplification. Agarose gel showing PCR products from amplification of specific plasmid fragments in the EV-enriched fractions. Lane 1: YEp352 plasmid. Lane 2: EV fraction from BY4741-YEp352 strain; Lane 3: negative control (no template) for YEp352 primers; Lane 4: pRS316 plasmid. Lane 5: EV fraction from BY4741-pRS316 strain; Lane 6: negative control for pRS316 primers; M: Molecular weight marker NZYDNA Ladder V (100-1,000 bp). The amplified region corresponds to the 2μ origin for YEp352 and to the CEN/ARS sequence for pRS316. Positions of molecular weight markers are indicated on the right (bp). PCR: Polymerase chain reaction; EV: extracellular vesicle.