fig5

Figure 5. MSC-EV immunophenotype. Representative cytograms of EV (CD9/63/81) and MSC (CD73/90) markers tested in representative MSC-EVs after flow cytometer calibration with FITC-positive polystyrene beads of predetermined size (100, 160, 200, 240, 300, 500 and 900 nm) to confirm reliability of particle detection in the nanometric range. CFSE-stained only and antibody-unstained samples represent EVs from ASCs for clarity. No significant statistical difference (P-value ≤ 0.05, N = 3) emerged for tested markers. After assessment of normality of data distribution by the Shapiro-Wilk test, statistical significance was determined using one-way ANOVA. Data presented in PowerPoint. MSC: Mesenchymal stromal cell; ASC: adipose-derived mesenchymal stromal cell; BMSC: bone marrow-derived mesenchymal stromal cell; hAMSC: human amniotic mesenchymal stromal cell; EVs: extracellular vesicles; ASC-EVs: extracellular vesicles derived from ASCs; BMSC-EVs: extracellular vesicles derived from BMSCs; hAMSC-EVs: extracellular vesicles derived from hAMSCs; CD: cluster of differentiation; FITC: fluorescein isothiocyanate; APC: allophycocyanin; CFSE: carboxyfluorescein succinimidyl ester; PBS: phosphate-buffered saline; ANOVA: analysis of variance; SSC: side scatter; nm: nanometer.