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Figure 1. Analysis of EV populations in synovial fluid with an extended antibody panel. Following staining with different antibodies that had been qualified for the single EV analyses, synovial fluid aliquots were analyzed by imaging flow cytometry. (A) Applied gating strategy; events with coincidences (multiplets) were excluded from analyses using the Spot Count feature within the AMNIS Imagestream software. Plots show events before and after gating, including a zoom-in of the plot with the gated events (B) Concentration of the EVs recognized after labeling with each of the 12 antibodies used in this study. EV populations were recorded in either two samples of synovial fluid of patients with aseptic and septic endoprosthesis loosening, respectively. Drawing Software: CorelDraw (2021, Version 23.1.0.389). (A) plots exported from the software (IDEAS), with axis font sizes adjusted in CorelDraw to enhance visibility; (B) GraphPad. (A) and (B) compiled with CorelDraw. EV: Extracellular vesicle; PJI: periprosthetic joint infection.