fig7

Figure 7. Effect of FGFR inhibitor BGJ398 on neuroblastoma cell proliferation and survival. (A) Neuroblastoma cell lines (SK-N-AS, SK-N-SH, SK-N-BE(2), IMR-32, NGP, SH-SY5Y, LA155N, NBL-S, CHP134, Kelly, CHP212, and NB-10) were treated with increasing concentrations of BGJ398 for 72 h. Mean average values for a reduction in confluence (% inhibition) from triplicate experiments were then plotted in Microsoft Excel against BGJ398 dose levels; (B) IC50 values were calculated using curve-fit equations for each tested neuroblastoma cell line listed from lowest to highest IC50 values for each cell type; (C) Relative FGFR1-4 gene expression levels in cell lines were obtained by RNA-sequencing and were graphed in Microsoft Excel as fragments per kb of transcript per million fragments mapped (FPKM); and (D) FGFR gene member expression was compared to calculated IC50 values using Python Code software. FGFR: fibroblast growth factor receptor; BGJ398: infigratinib.