Acquisition of drug resistance in endothelial cells by tumor-derived extracellular vesicles and cancer progression
Abstract
Angiogenesis by endothelial cells (ECs) is essential for tumor growth. Angiogenesis inhibitors are used in combination with anticancer drugs in many tumor types, but tumors eventually become resistant. Previously, the underlying mechanism for developing drug resistance was considered to be a change in the characteristics of tumor cells whereas ECs were thought to be genetically stable and do not contribute to drug resistance. However, tumor endothelial cells (TECs) have been shown to differ from normal endothelial cells (NECs) in that they exhibit chromosomal abnormalities, angiogenic potential, and drug resistance. Extracellular vesicles (EVs) secreted by tumor cells have recently attracted attention as a factor involved in the acquisition of such abnormalities. Various cells communicate with each other through EVs, and it has been reported that tumor-derived EVs act on other tumor cells or stromal cells to develop drug resistance. Drug-resistant tumor cells confer drug resistance to recipient cells by transporting mRNAs encoding ATP-binding cassette subfamily B member 1 (ABCB1) and ATP-binding cassette subfamily C member 1 (ABCC1) as well as miRNAs involved in signaling such as Akt, drug efflux transporters, and P-glycoprotein modulators via EVs. However, there are limited reports on the acquisition of drug resistance in ECs by tumor-derived EVs. Since drug resistance of ECs may induce tumor metastasis and support tumor cell proliferation, the mechanism underlying the development of resistance should be elucidated to find therapeutic application. This review provides insight into the acquisition of drug resistance in ECs via tumor EVs in the tumor microenvironment.
Keywords
INTRODUCTION
Tumor angiogenesis and antiangiogenic therapy
Angiogenesis refers to the sprouting and elongation of new vascular branches from existing vessels, and vasculogenesis refers to the process by which vascular endothelial cells (ECs) differentiate from vascular progenitor cells to form a lumen[1]. Angiogenesis is induced in ischemic tissues, such as tumors and wound healing tissue, when cells release angiogenic factors such as vascular endothelial growth factors (VEGF) under the influence of hypoxia.
Angiogenesis by ECs is essential because tumor growth requires the supply of nutrients and oxygen from blood vessels[2]. Tumor blood vessels also provide a route for tumor cells to metastasize to distant organs[3,4]. Therefore, tumor blood vessels have been recognized as an important target for cancer treatment since Dr. Folkman proposed the idea that tumor growth depends on angiogenesis in 1971[5]. Antiangiogenic therapy blocks angiogenesis, a common process in tumor growth. The first angiogenesis inhibitor was bevacizumab, a monoclonal antibody against human VEGF. In addition, sorafenib, sunitinib, and pazopanib, which inhibit the VEGF receptor (VEGFR) tyrosine kinase, have been developed for cancer treatment[6]. Angiogenesis inhibitors also normalize immature, leaky, and dysfunctional tumor blood vessels and improve the delivery of drugs and immune cells to tumor tissues[7,8]. Thus, angiogenesis inhibitors displayed an additive effect when combined with anticancer drugs such as 5-FU, cisplatin, and gemcitabine in many tumor types[9]. However, existing angiogenic inhibitors, such as bevacizumab, block VEGF signaling, which is also essential for normal blood vessels. These drugs may cause side effects such as hypertension and bleeding[10].
The abnormalities and drug resistance of tumor endothelial cells
Tumor blood vessels are histo-pathologically different from normal vessels[11]. Vascular permeability is increased due to sparse adhesion between ECs and between mural cells and ECs[12]. The vascular basement membrane is also abnormal, showing intermittent vessel bending, which may cause staggered blood flow[8]. In other words, tumor vessels are heterogeneous and disorganized in contrast to normal blood vessels that have an ordered, hierarchical structure[13]. Therefore, despite the abundance of blood vessels, blood flow is low and cancer tissue is hypoxic[14].
Angiogenesis inhibitors used in combination with anticancer drugs are effective in the early stages, but tumors eventually become resistant, which limits the long-term therapeutic use of these drugs. One of the advantages of targeting ECs rather than tumor cells is that, unlike tumor cells, which are genetically unstable, ECs were thought to be genetically stable and do not acquire drug resistance until recently[15]. The mechanism underlying the development of resistance has been considered to be a change in the characteristics of tumor cells. These include the acquisition of anticancer drug resistance by tumor cells[16] and the increased production of angiogenic factors other than VEGF [e.g., fibroblast growth factor (FGF)] as a compensatory response to VEGF inhibition[17]. However, in the tumor microenvironment, tumor endothelial cells (TECs) are exposed to hypoxia, nutrient deprivation, and cytokines secreted by tumor cells and tumor stromal cells. In other words, TECs exist in a very different environment compared to normal endothelial cells (NECs). These are all known as mechanisms by which cancer cells acquire drug resistance. In fact, TECs exhibit a variety of abnormalities[18]. We found that TECs have various abnormalities compared to NECs, including chromosomal abnormalities[19,20], increased angiogenic potential[21], and elevated expression of genes such as cyclooxygenase-2[22], VEGF[23], CXCR7[24,25], biglycan[26-28], lysyl oxidase[29], lectin-like ox-LDL receptor 1[30], and PTGIR[31]. In addition, we have reported that tumor cell-secreted VEGF and other factors upregulate the expression of MDR1/ATP-binding cassette subfamily B member 1 (ABCB1), a stem cell marker, in TECs. The ABC transporter P-glycoprotein (P-gp), a transcript of MDR1/ABCB1, promotes drug efflux[32,33]. We also found that inflammatory changes in tumor tissues during anticancer therapy, such as increased IL-8 production, induce MDR1/ABCB1 expression in TECs, resulting in resistance to paclitaxel, which is mediated by ABCB1[34]. Naito et al. reported the existence of stem cell-like TECs that express high levels of P-gp, a stem cell marker, and are resistant to tyrosine kinase inhibitors that primarily target VEGFR[35]. We found that the stem cell markers aldehyde dehydrogenase (ALDH), Sca-1, CD90, and MDR1 were highly expressed in TECs, indicating drug resistance[36]. Furthermore, TEC in renal carcinoma was shown to be resistant to vincristine[37] while TEC in hepatocellular carcinoma is resistant to 5-FU[38]. These findings suggest that TECs that have acquired stem cell-like properties in the tumor microenvironment are likely to survive after chemotherapy and maintain tumor cell proliferation. Therefore, understanding the properties and the mechanisms of characteristic changes of TECs is very important to study the effectiveness of drug treatment. It has been reported that drug resistance, chromosomal aberrations, and stemness of TECs differ depending on tumor malignancy[39]. These results suggest that factors derived from the tumor microenvironment may induce abnormalities in ECs. Paracrine effects of tumor microenvironment factors, such as tumor cell-derived cytokines, on ECs have been reported[40]. It was also suggested that hypoxia may cause abnormalities in ECs[41]. Furthermore, tumor cell-derived extracellular vesicles (EVs) may be involved in the development of drug resistance in ECs.
EFFECTS OF TUMOR EVS ON STROMAL CELLS IN THE TUMOR MICROENVIRONMENT
EVs as a tool for intercellular communication
EVs secreted by tumor cells have attracted attention as tumor microenvironment factors. EVs are vesicles with lipid bilayer membranes that are 50-1,000 nm in diameter and secreted by various cells[42]. They have been called exosomes, ectosomes, microvesicles, shedding vesicles, etc., depending on their size and origin[42]. Exosomes are endosomal membrane-derived vesicles formed by endocytosis processes at
EVs differ from factors involved in intercellular communication, such as cytokines and extracellular matrix, in that they are complexes of different substances[43]. Information from the source cell is packaged into EVs and taken up by the recipient cell to exert various effects[48]. EVs secreted by tumor cells are known to act on the tumor itself and the tumor microenvironment to influence its growth and metastasis[49]. The contents of EVs exhibit characteristics of the cells from which they are derived. Therefore, EVs circulating in body fluids are used as liquid biopsies and are recognized as biomarkers for early detection, diagnosis, treatment, and response to treatment in cancer patients.
Effects of tumor EVs on stromal cells
Stromal cells play an important role in the tumor microenvironment. The stromal cells are composed of vascular and lymphatic ECs, macrophages, dendritic cells, lymphocytes, neurocytes, fibroblasts, and adipocytes. It has been reported that tumor cells act on these stromal cells by secreting EVs to create an environment conducive to their own growth and metastasis. For example, miR-146a-5p in EVs secreted by osteosarcoma cells is taken up by macrophages (preosteoclasts) and inhibits differentiation into osteoclasts, promoting tumor invasion and metastasis[50]. miR-1247-3p in EVs secreted by highly metastatic hepatocellular carcinoma cells directly targets B4GALT3 in fibroblasts and activates β1-integrin-NF-κB signaling. As a result, inflammatory cytokines such as IL-6 and IL-8 are secreted, promoting lung metastasis[51]. Colorectal cancer-derived integrin beta-like 1 (ITGBL1)-rich EVs stimulate the NF-κB signaling pathway via tumor necrosis factor (TNF) alpha-induced protein 3 (TNFAIP3) to activate fibroblasts at metastatic sites and promote cancer metastasis[52]. EVs released from glioma cells, including Wilms tumor-1 (WT1), are taken up by microglia and downregulate the expression of thrombospondin-1, a negative regulator of angiogenesis. As a result, angiogenesis is promoted by microglia[53].
Promotion of angiogenesis by tumor EVs
Tumor-derived EVs also act on ECs [Figure 1], and many reports have shown that they enhance angiogenic potential in particular. For example, EVs derived from human squamous cell carcinoma cells transport epidermal growth factor receptor (EGFR) to ECs and enhance their angiogenic potential[54]. In addition, vascular endothelial growth factor A (VEGF-A)[55], matrix metalloproteinase 13[56], and vasorin[57] in tumor-derived EVs are transported to ECs and enhance their angiogenic potential.
Figure 1. Tumor-derived EVs exert various effects on ECs. Tumor-derived EV cargo molecules such as EGFR, VEGF-A, MMP13, VASN, miR-9, miR-205, and miR-210 are transported to ECs to induce the proangiogenic phenotype. miR-105, miR-181c, and miR-939 attenuate endothelial adhesion. miR-23a and miR-25-3p induce angiogenesis and disrupt the EC barrier, while TrpC5 and P-gp confer drug resistance to ECs. In addition, miR-1246 promotes tumor cell adhesion to ECs, disrupts the EC barrier, and confers drug resistance. EVs: Extracellular vesicles; ECs: endothelial cells; EGFR: epidermal growth factor receptor; VEGF-A: vascular endothelial growth factor A; MMP13: matrix metalloproteinase 13; VASN: vasorin.
miRNAs are also important as EV cargoes that induce angiogenesis. miR-210 in EVs derived from breast cancer[58] or hypoxic leukemia cells[59], and miR-9 in glioma EVs[60] are transported to ECs and induce angiogenesis. In addition, we reported that tumor cell-derived EVs were incorporated into EC and enhanced their angiogenic potential via Akt activation[61].
Pre-metastatic niche formation by tumor EV
Furthermore, since ECs act as gatekeepers for tumor metastasis, altering their characteristics may promote metastasis. In particular, it has been reported that targeting adhesion molecules between ECs, such as
ACQUISITION OF DRUG RESISTANCE BY TUMOR EVS
Induction of drug efflux pump expression by tumor EVs
Tumor-derived EVs are also closely associated with drug resistance[68] [Figure 2]. There have been several cases in which tumor cells resistant to anticancer drugs confer drug resistance to sensitive cells via EVs. Qin et al. have reported that EVs derived from cisplatin-resistant lung cancer cells confer resistance to other sensitive cells[69]. In this study, they analyzed miRNA expression profiles of EVs derived from cisplatin-resistant cells and those from sensitive cancer cells, respectively. They focused on miR-100-5p, which is most downregulated in EVs derived from resistant lung cancer cells, and identified the mammalian target of rapamycin (mTOR) as a target gene using several bioinformatics methods. Using miR-100-5p mimics and inhibitors, they found that EVs derived from cisplatin-resistant cancer cells confer drug resistance via increased mTOR expression both in vitro and in vivo.
Figure 2. Drug resistance is conferred to other cells through tumor-derived EVs. Drug-resistant tumor cells confer drug resistance to recipient cells by transporting mRNAs for ABCB1 and ABCC1, miRNAs involved in signaling such as Akt, and drug efflux transporters and P-gp modulators via EVs. EVs: Extracellular vesicles; ABCB1: ATP-binding cassette subfamily B member 1; ABCC1: ATP-binding cassette subfamily C member 1; P-gp: P-glycoprotein.
In addition, major proteins involved in the acquisition of multidrug resistance in tumor cells include P-gp/ABCB1, multidrug resistance-related protein1 [MRP1/ATP-binding cassette subfamily C member 1 (ABCC1)], and breast cancer resistance protein (BCRP/ABCG2)[70]. Increased expression of these transporters responsible for drug efflux in tumor cells promotes drug excretion, resulting in drug resistance. It has been reported that EVs secreted by drug-resistant tumor cells induce the expression of drug efflux pumps in drug-sensitive tumor cells, leading to drug resistance. EVs released from docetaxel-resistant tumor cells transport P-gp to sensitive cells and confer drug resistance[71,72].
Acquisition of drug resistance via EV-mediated packaging
Drug-resistant tumor cells are known to shed more EVs than drug-sensitive cells[73]. It has also been shown that exposure of tumor cells to therapeutic drugs promotes drug resistance by packaging drugs into EVs and releasing them outside the cell[74]. Rab27B is highly expressed in 5-FU-resistant hepatocellular carcinoma cells, which secrete large amounts of EVs upon 5-FU stimulation. Rab27B knockdown decreased EV secretion and increased intracellular accumulation of 5-FU[75]. Drug-resistant oral squamous cell carcinoma cells produce more EVs than drug-sensitive cells, and high levels of cisplatin are detected in the EVs. Inhibition of EV secretion by proton pump inhibitors resulted in increased drug sensitivity in cisplatin-resistant cells[76]. Exposure of multiple myeloma cells to the therapeutic drug lenalidomide has been shown to increase SORT1/LAMP2 expression and EV secretion, which in turn causes sensitive cells to acquire tolerance[77]. As shown above, drug packaging by EVs is one of the mechanisms for drug resistance, and research looking for its therapeutic application is currently ongoing.
Acquisition of drug resistance in ECs by tumor EVs
Although it was thought that resistance to antiangiogenic therapy is primarily due to phenotypic changes of the tumor cells, as mentioned above, ECs also acquire drug resistance. Crosstalk between tumor cells and stromal cells is an important factor in tumor progression, and evidence is accumulating to support the idea that tumor cell-derived EVs are associated with drug resistance of ECs. Drug resistance of the TECs that make up tumor blood vessels means that pathways for nutrient and oxygen delivery to tumor cells and for metastasis remain. We have shown that VEGF-A induces MDR1/ABCB1 expression in NECs through activation of Y-box binding protein 1[32]. TECs were resistant to 5-FU, which is not a substrate of ABCB1[39], suggesting that other mechanisms of drug resistance exist in TECs. We found elevations of IL-6 expression and Akt activation in TECs compared to NECs[36], suggesting that tumor-derived EVs may act on this pathway as a tumor microenvironment factor. We analyzed miRNAs in EVs from high and low metastatic melanoma and identified that miR-1246 is abundant in EVs from highly metastatic melanoma[78]. Since IL-6 was not detected in the tumor-derived conditioned medium[78], we hypothesized that the IL-6 autocrine loop in ECs receiving EVs was responsible for resistance. We then revealed the mechanism by which highly metastatic melanoma-derived EVs are taken up by ECs. In addition, miR-1246 targets the androgen receptor to increase IL-6 expression, leading to drug resistance via phosphorylation of Akt and signal transducer and activator of transcription 3 (STAT3)[78] [Figure 3]. Dong et al. reported that TrpC5 and P-gp in breast cancer-derived EVs are transported to ECs, suggesting a mechanism of EC resistance[79]. However, drug resistance of ECs induced by tumor-derived EVs has not been reported elsewhere. Table 1 shows the reports of EC drug resistance by tumor EVs identified to date. Since EVs are complexes containing proteins and nucleic acids, it is expected that ECs may exhibit drug resistance by receiving drug efflux pumps and miRNAs that regulate molecular expression, but further analysis is needed.
Figure 3. Drug resistance of ECs by tumor-derived EVs. Metastatic melanoma-derived EVs are taken up by ECs. Then, miR-1246 enhances IL-6 expression by targeting AR. An autocrine system is utilized to induce drug resistance via phosphorylation of Akt and activation of STAT3. This figure is a modification of the schema in[78]. ECs: Endothelial cells; EVs: extracellular vesicles; AR: androgen receptor; STAT3: signal transducer and activator of transcription 3.
Reports on drug resistance of ECs by tumor-derived EVs
| Donor cell | Recipient cell | Relevant EV cargo | Target gene | Mechanism | Ref. |
| A375SM cells | HMVECs | miR-1246 | Androgen receptor | miR-1246 increases IL-6 expression by targeting the androgen receptor and activating Akt and STAT3 | [78] |
| Adriamycin-resistant MCF-7 cells | HMECs | TrpC5, P-gp | - | TrpC5 incorporated into EC induces P-gp expression via translocation of NFATc3 | [79] |
Effects of TEC-derived EVs
Conversely, the effects of EVs derived from tumor stromal cells on tumor cells have recently been reported. EVs derived from cancer-associated fibroblasts (CAFs) have been shown to induce Snail expression, pancreatic cancer cell proliferation, and drug resistance[80]. Tumor-associated macrophage (TAM)-derived EVs were taken up by lung adenocarcinoma cells, and miR-942 was shown to promote the ability of invasion and migration by suppressing FOXO1 expression[81].
There are also a few reports describing the effects of EC-derived EVs on tumor cells. miR-203 in human umbilical vein endothelial cell (HUVEC)-derived EVs inhibits the progression of non-small cell lung cancer (NSCLC) by targeting DTL and increasing the stability of p21, a tumor suppressor[82]. Lombardo et al. showed that IL-3 promotes EV release in ECs isolated from human umbilical veins and that miR-126-3p and pSTAT5 within these EVs induce angiogenesis in other ECs[83]. They found that a treatment with
Recently, TEC-derived EVs have been reported to inhibit tumor immunity. TEC-derived EVs from head and neck cancer acted on peripheral blood mononuclear cells (PBMCs) to modulate cytokine secretion and stimulate T regulatory cell (Treg) formation, thereby causing immunosuppression[86]. Some TEC-specific markers, such as biglycan, activated NF-κB, a transcription factor for the immune checkpoint molecule
Currently, there are few reports on the effects of TEC-derived EVs, especially on tumor cells, and further research is needed.
PROSPECTS FOR DRUG THERAPY FROM EV RESEARCH
Understanding the mechanisms of drug resistance of ECs via tumor-derived EVs or ECs themselves will lead to the development of new therapeutic strategies targeting both tumors and ECs to improve the outcome of patients with resistant tumors. Nishida-Aoki et al. have shown that the removal of tumor-derived EVs by intravenous administration of antibodies to tumor-bearing mice suppresses cancer metastasis[87], suggesting a possibility of its therapeutic application. However, a method to target EVs for treatment has not yet been established for clinical uses. Discovery of a marker specific for EVs secreted by tumors and TECs will lead to an effective therapy.
In addition, recent evidence suggests that autophagy may contribute to the acquisition of drug resistance in ECs. Regarding bortezomib resistance in multiple myeloma, the combination of bortezomib, a proteasome inhibitor, and hydroxychloroquine, an autophagy inhibitor, was shown to promote autophagy of ECs and inhibit myeloma plasma cell growth[88]. These results demonstrate the efficacy of bortezomib in combination with an autophagy inhibitor in the treatment of resistant multiple myeloma, and indicate the necessity of using an angiogenesis inhibitor to achieve a complete remission. Therefore, we speculate that future research should focus on autophagy to further understand the mechanism underlying drug resistance of ECs.
CONCLUSION
In the tumor microenvironment, EVs play an important role as signaling molecules between tumor cells and stromal cells. Tumor cell-derived EVs induce TEC-like characteristics in NECs, such as angiogenic potential and drug resistance. Overcoming drug resistance in TECs is crucial to improve therapeutic efficacy, since drug-resistant TECs promote tumor metastasis and support tumor cell proliferation. It is desirable to develop new therapies that target tumors and specifically TEC-derived EVs, while also elucidating the mechanisms underlying the acquisition of resistance.
DECLARATIONS
Acknowledgments
We thank the members of the Vascular Biology and Molecular Pathology, Hokkaido University Faculty of Dental Medicine, for helpful discussions.
Authors’ contributions
Writing-original draft: Morimoto M
Writing-review and editing: Maishi N
Conceptualization, supervision, writing-review, and editing: Hida K
All authors have read and agreed to the published version of the manuscript.
Availability of data and materials
Not applicable.
Financial support and sponsorship
This research was supported by JSPS Grants-in-Aid for Scientific Research to NM (JP18K09715) and KH (JP18H02996).
Conflict of interest
All authors declare that there are no conflicts of interest.
Ethical approval and consent to participate
Not applicable.
Consent for publication
Not applicable.
Copyright
© The Author(s) 2024.
REFERENCES
1. Folkman J. Angiogenesis: an organizing principle for drug discovery? Nat Rev Drug Discov 2007;6:273-86.
4. Reymond N, d’Água BB, Ridley AJ. Crossing the endothelial barrier during metastasis. Nat Rev Cancer 2013;13:858-70.
6. Ivy SP, Wick JY, Kaufman BM. An overview of small-molecule inhibitors of VEGFR signaling. Nat Rev Clin Oncol 2009;6:569-79.
7. Viallard C, Larrivée B. Tumor angiogenesis and vascular normalization: alternative therapeutic targets. Angiogenesis 2017;20:409-26.
8. Jain RK. Normalizing tumor vasculature with anti-angiogenic therapy: a new paradigm for combination therapy. Nat Med 2001;7:987-9.
9. Ohtsu A, Shah MA, Van Cutsem E, et al. Bevacizumab in combination with chemotherapy as first-line therapy in advanced gastric cancer: a randomized, double-blind, placebo-controlled phase III study. J Clin Oncol 2011;29:3968-76.
10. Burger RA. Experience with bevacizumab in the management of epithelial ovarian cancer. J Clin Oncol 2007;25:2902-8.
11. Pasqualini R, Arap W, McDonald DM. Probing the structural and molecular diversity of tumor vasculature. Trends Mol Med 2002;8:563-71.
12. McDonald DM, Baluk P. Significance of blood vessel leakiness in cancer. Cancer Res 2002;62:5381-5.
13. Fidler IJ. Angiogenic heterogeneity: regulation of neoplastic angiogenesis by the organ microenvironment. J Natl Cancer Inst 2001;93:1040-1.
14. Muz B, de la Puente P, Azab F, Azab AK. The role of hypoxia in cancer progression, angiogenesis, metastasis, and resistance to therapy. Hypoxia 2015;3:83-92.
15. Kerbel RS. Inhibition of tumor angiogenesis as a strategy to circumvent acquired resistance to anti-cancer therapeutic agents. Bioessays 1991;13:31-6.
17. Casanovas O, Hicklin DJ, Bergers G, Hanahan D. Drug resistance by evasion of antiangiogenic targeting of VEGF signaling in late-stage pancreatic islet tumors. Cancer Cell 2005;8:299-309.
18. Langenkamp E, Molema G. Microvascular endothelial cell heterogeneity: general concepts and pharmacological consequences for anti-angiogenic therapy of cancer. Cell Tissue Res 2009;335:205-22.
19. Hida K, Hida Y, Amin DN, et al. Tumor-associated endothelial cells with cytogenetic abnormalities. Cancer Res 2004;64:8249-55.
20. Akino T, Hida K, Hida Y, et al. Cytogenetic abnormalities of tumor-associated endothelial cells in human malignant tumors. Am J Pathol 2009;175:2657-67.
21. Matsuda K, Ohga N, Hida Y, et al. Isolated tumor endothelial cells maintain specific character during long-term culture. Biochem Biophys Res Commun 2010;394:947-54.
22. Muraki C, Ohga N, Hida Y, et al. Cyclooxygenase-2 inhibition causes antiangiogenic effects on tumor endothelial and vascular progenitor cells. Int J Cancer 2012;130:59-70.
23. Kurosu T, Ohga N, Hida Y, et al. HuR keeps an angiogenic switch on by stabilising mRNA of VEGF and COX-2 in tumour endothelium. Br J Cancer 2011;104:819-29.
24. Maishi N, Ohga N, Hida Y, et al. CXCR7: a novel tumor endothelial marker in renal cell carcinoma. Pathol Int 2012;62:309-17.
25. Yanagiya M, Dawood RIH, Maishi N, et al. Correlation between endothelial CXCR7 expression and clinicopathological factors in oral squamous cell carcinoma. Pathol Int 2021;71:383-91.
26. Maishi N, Ohba Y, Akiyama K, et al. Tumour endothelial cells in high metastatic tumours promote metastasis via epigenetic dysregulation of biglycan. Sci Rep 2016;6:28039.
27. Yamamoto K, Ohga N, Hida Y, et al. Biglycan is a specific marker and an autocrine angiogenic factor of tumour endothelial cells. Br J Cancer 2012;106:1214-23.
28. Cong L, Maishi N, Annan DA, et al. Inhibition of stromal biglycan promotes normalization of the tumor microenvironment and enhances chemotherapeutic efficacy. Breast Cancer Res 2021;23:51.
29. Osawa T, Ohga N, Akiyama K, et al. Lysyl oxidase secreted by tumour endothelial cells promotes angiogenesis and metastasis. Br J Cancer 2013;109:2237-47.
30. Tsumita T, Maishi N, Annan DAM, et al. The oxidized-LDL/LOX-1 axis in tumor endothelial cells enhances metastasis by recruiting neutrophils and cancer cells. Int J Cancer 2022;151:944-56.
31. Osawa T, Ohga N, Hida Y, et al. Prostacyclin receptor in tumor endothelial cells promotes angiogenesis in an autocrine manner. Cancer Sci 2012;103:1038-44.
32. Akiyama K, Ohga N, Hida Y, et al. Tumor endothelial cells acquire drug resistance by MDR1 up-regulation via VEGF signaling in tumor microenvironment. Am J Pathol 2012;180:1283-93.
33. Akiyama K, Maishi N, Ohga N, et al. Inhibition of multidrug transporter in tumor endothelial cells enhances antiangiogenic effects of low-dose metronomic paclitaxel. Am J Pathol 2015;185:572-80.
34. Kikuchi H, Maishi N, Annan DA, et al. Chemotherapy-induced IL8 upregulates MDR1/ABCB1 in tumor blood vessels and results in unfavorable outcome. Cancer Res 2020;80:2996-3008.
35. Naito H, Wakabayashi T, Kidoya H, et al. Endothelial side population cells contribute to tumor angiogenesis and antiangiogenic drug resistance. Cancer Res 2016;76:3200-10.
36. Ohmura-Kakutani H, Akiyama K, Maishi N, et al. Identification of tumor endothelial cells with high aldehyde dehydrogenase activity and a highly angiogenic phenotype. PLoS One 2014;9:e113910.
37. Bussolati B, Deambrosis I, Russo S, Deregibus MC, Camussi G. Altered angiogenesis and survival in human tumor-derived endothelial cells. FASEB J 2003;17:1159-61.
38. Xiong YQ, Sun HC, Zhang W, et al. Human hepatocellular carcinoma tumor-derived endothelial cells manifest increased angiogenesis capability and drug resistance compared with normal endothelial cells. Clin Cancer Res 2009;15:4838-46.
39. Ohga N, Ishikawa S, Maishi N, et al. Heterogeneity of tumor endothelial cells: comparison between tumor endothelial cells isolated from high- and low-metastatic tumors. Am J Pathol 2012;180:1294-307.
40. Sheu BC, Chang WC, Cheng CY, Lin HH, Chang DY, Huang SC. Cytokine regulation networks in the cancer microenvironment. Front Biosci 2008;13:6255-68.
41. Kondoh M, Ohga N, Akiyama K, et al. Hypoxia-induced reactive oxygen species cause chromosomal abnormalities in endothelial cells in the tumor microenvironment. PLoS One 2013;8:e80349.
42. Jeppesen DK, Zhang Q, Franklin JL, Coffey RJ. Extracellular vesicles and nanoparticles: emerging complexities. Trends Cell Biol 2023;33:667-81.
43. Valadi H, Ekström K, Bossios A, Sjöstrand M, Lee JJ, Lötvall JO. Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Nat Cell Biol 2007;9:654-9.
44. Todorova D, Simoncini S, Lacroix R, Sabatier F, Dignat-George F. Extracellular vesicles in angiogenesis. Circ Res 2017;120:1658-73.
45. Raposo G, Stoorvogel W. Extracellular vesicles: exosomes, microvesicles, and friends. J Cell Biol 2013;200:373-83.
46. Chen J, Li P, Zhang T, et al. Review on strategies and technologies for exosome isolation and purification. Front Bioeng Biotechnol 2021;9:811971.
47. Théry C, Witwer KW, Aikawa E, et al. Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines. J Extracell Vesicles 2018;7:1535750.
48. Forder A, Hsing CY, Trejo Vazquez J, Garnis C. Emerging role of extracellular vesicles and cellular communication in metastasis. Cells 2021;10:3429.
49. Bobrie A, Krumeich S, Reyal F, et al. Rab27a supports exosome-dependent and -independent mechanisms that modify the tumor microenvironment and can promote tumor progression. Cancer Res 2012;72:4920-30.
50. Araki Y, Aiba H, Yoshida T, et al. Osteosarcoma-derived small extracellular vesicles enhance tumor metastasis and suppress osteoclastogenesis by miR-146a-5p. Front Oncol 2021;11:667109.
51. Fang T, Lv H, Lv G, et al. Tumor-derived exosomal miR-1247-3p induces cancer-associated fibroblast activation to foster lung metastasis of liver cancer. Nat Commun 2018;9:191.
52. Ji Q, Zhou L, Sui H, et al. Primary tumors release ITGBL1-rich extracellular vesicles to promote distal metastatic tumor growth through fibroblast-niche formation. Nat Commun 2020;11:1211.
53. Tsutsui T, Kawahara H, Kimura R, et al. Glioma-derived extracellular vesicles promote tumor progression by conveying WT1. Carcinogenesis 2020;41:1238-45.
54. Al-Nedawi K, Meehan B, Kerbel RS, Allison AC, Rak J. Endothelial expression of autocrine VEGF upon the uptake of tumor-derived microvesicles containing oncogenic EGFR. Proc Natl Acad Sci U S A 2009;106:3794-9.
55. Treps L, Perret R, Edmond S, Ricard D, Gavard J. Glioblastoma stem-like cells secrete the pro-angiogenic VEGF-A factor in extracellular vesicles. J Extracell Vesicles 2017;6:1359479.
56. You Y, Shan Y, Chen J, et al. Matrix metalloproteinase 13-containing exosomes promote nasopharyngeal carcinoma metastasis. Cancer Sci 2015;106:1669-77.
57. Huang A, Dong J, Li S, et al. Exosomal transfer of vasorin expressed in hepatocellular carcinoma cells promotes migration of human umbilical vein endothelial cells. Int J Biol Sci 2015;11:961-9.
58. Kosaka N, Iguchi H, Hagiwara K, Yoshioka Y, Takeshita F, Ochiya T. Neutral sphingomyelinase 2 (nSMase2)-dependent exosomal transfer of angiogenic microRNAs regulate cancer cell metastasis. J Biol Chem 2013;288:10849-59.
59. Tadokoro H, Umezu T, Ohyashiki K, Hirano T, Ohyashiki JH. Exosomes derived from hypoxic leukemia cells enhance tube formation in endothelial cells. J Biol Chem 2013;288:34343-51.
60. Chen X, Yang F, Zhang T, et al. MiR-9 promotes tumorigenesis and angiogenesis and is activated by MYC and OCT4 in human glioma. J Exp Clin Cancer Res 2019;38:99.
61. Kawamoto T, Ohga N, Akiyama K, et al. Tumor-derived microvesicles induce proangiogenic phenotype in endothelial cells via endocytosis. PLoS One 2012;7:e34045.
62. Zhou W, Fong MY, Min Y, et al. Cancer-secreted miR-105 destroys vascular endothelial barriers to promote metastasis. Cancer Cell 2014;25:501-15.
63. Zeng Z, Li Y, Pan Y, et al. Cancer-derived exosomal miR-25-3p promotes pre-metastatic niche formation by inducing vascular permeability and angiogenesis. Nat Commun 2018;9:5395.
64. Tominaga N, Kosaka N, Ono M, et al. Brain metastatic cancer cells release microRNA-181c-containing extracellular vesicles capable of destructing blood-brain barrier. Nat Commun 2015;6:6716.
65. Di Modica M, Regondi V, Sandri M, et al. Breast cancer-secreted miR-939 downregulates VE-cadherin and destroys the barrier function of endothelial monolayers. Cancer Lett 2017;384:94-100.
66. Hsu YL, Hung JY, Chang WA, et al. Hypoxic lung cancer-secreted exosomal miR-23a increased angiogenesis and vascular permeability by targeting prolyl hydroxylase and tight junction protein ZO-1. Oncogene 2017;36:4929-42.
67. Morimoto M, Maishi N, Tsumita T, et al. miR-1246 in tumor extracellular vesicles promotes metastasis via increased tumor cell adhesion and endothelial cell barrier destruction. Front Oncol 2023;13:973871.
68. Sharma A. Chemoresistance in cancer cells: exosomes as potential regulators of therapeutic tumor heterogeneity. Nanomedicine 2017;12:2137-48.
69. Qin X, Yu S, Zhou L, et al. Cisplatin-resistant lung cancer cell-derived exosomes increase cisplatin resistance of recipient cells in exosomal miR-100-5p-dependent manner. Int J Nanomedicine 2017;12:3721-33.
70. Schinkel AH, Jonker JW. Mammalian drug efflux transporters of the ATP binding cassette (ABC) family: an overview. Adv Drug Deliv Rev 2012;64:138-53.
71. Corcoran C, Rani S, O’Brien K, et al. Docetaxel-resistance in prostate cancer: evaluating associated phenotypic changes and potential for resistance transfer via exosomes. PLoS One 2012;7:e50999.
72. Lv M, Zhu X, Chen W, et al. Exosomes mediate drug resistance transfer in MCF-7 breast cancer cells and a probable mechanism is delivery of P-glycoprotein. Tumour Biol 2014;35:10773-9.
73. Lopes-Rodrigues V, Di Luca A, Sousa D, et al. Multidrug resistant tumour cells shed more microvesicle-like EVs and less exosomes than their drug-sensitive counterpart cells. Biochim Biophys Acta 2016;1860:618-27.
74. Wang J, Yeung BZ, Cui M, et al. Exosome is a mechanism of intercellular drug transfer: application of quantitative pharmacology. J Control Release 2017;268:147-58.
75. Li R, Dong C, Jiang K, et al. Rab27B enhances drug resistance in hepatocellular carcinoma by promoting exosome-mediated drug efflux. Carcinogenesis 2020;41:1583-91.
76. Khoo XH, Paterson IC, Goh BH, Lee WL. Cisplatin-resistance in oral squamous cell carcinoma: regulation by tumor cell-derived extracellular vesicles. Cancers 2019;11:1166.
77. Yamamoto T, Nakayama J, Yamamoto Y, Kuroda M, Hattori Y, Ochiya T. SORT1/LAMP2-mediated extracellular vesicle secretion and cell adhesion are linked to lenalidomide resistance in multiple myeloma. Blood Adv 2022;6:2480-95.
78. Torii C, Maishi N, Kawamoto T, et al. miRNA-1246 in extracellular vesicles secreted from metastatic tumor induces drug resistance in tumor endothelial cells. Sci Rep 2021;11:13502.
79. Dong YP, Pan QX, Jiang L, et al. Tumor endothelial expression of P-glycoprotein upon microvesicular transfer of TrpC5 derived from adriamycin-resistant breast cancer cells. Biochem Biophys Res Commun 2014;446:85-90.
80. Richards KE, Zeleniak AE, Fishel ML, Wu J, Littlepage LE, Hill R. Cancer-associated fibroblast exosomes regulate survival and proliferation of pancreatic cancer cells. Oncogene 2017;36:1770-8.
81. Wei K, Ma Z, Yang F, et al. M2 macrophage-derived exosomes promote lung adenocarcinoma progression by delivering miR-942. Cancer Lett 2022;526:205-16.
82. Ma T, Hu Y, Guo Y, Zhang Q. Human umbilical vein endothelial cells-derived microRNA-203-containing extracellular vesicles alleviate non-small-cell lung cancer progression through modulating the DTL/p21 axis. Cancer Gene Ther 2022;29:87-100.
83. Lombardo G, Dentelli P, Togliatto G, et al. Activated stat5 trafficking via endothelial cell-derived extracellular vesicles controls IL-3 pro-angiogenic paracrine action. Sci Rep 2016;6:25689.
84. Lombardo G, Gili M, Grange C, et al. IL-3R-alpha blockade inhibits tumor endothelial cell-derived extracellular vesicle (EV)-mediated vessel formation by targeting the β-catenin pathway. Oncogene 2018;37:1175-91.
85. Lopatina T, Grange C, Cavallari C, et al. Targeting IL-3Rα on tumor-derived endothelial cells blunts metastatic spread of triple-negative breast cancer via extracellular vesicle reprogramming. Oncogenesis 2020;9:90.
86. Lopatina T, Favaro E, Danilova L, et al. Extracellular vesicles released by tumor endothelial cells spread immunosuppressive and transforming signals through various recipient cells. Front Cell Dev Biol 2020;8:698.
87. Nishida-Aoki N, Tominaga N, Kosaka N, Ochiya T. Altered biodistribution of deglycosylated extracellular vesicles through enhanced cellular uptake. J Extracell Vesicles 2020;9:1713527.
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Morimoto M, Maishi N, Hida K. Acquisition of drug resistance in endothelial cells by tumor-derived extracellular vesicles and cancer progression. Cancer Drug Resist. 2024;7:1. http://dx.doi.org/10.20517/cdr.2023.121
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