fig3

Figure 3. TRAIL Receptor endocytosis. Death receptors are constitutively recycled by clathrin mediated endocytosis. Lack of death receptors leads to TRAIL resistance. After endocytosis of the receptor, clathrin and adaptor proteins (AP2) are recycled to the cell membrane to continue with more endocytosis of the receptors. Pharmacological inhibitors like PAO or chlorpromazine prevent the arrangement of clathrin and AP2 at the vesicles, hence prevent endocytosis, resulting in persistent expression of the death receptors at the surface and increased apoptosis.