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Figure 2. Altered expression of HDAC6 and HDAC7 among class II and IV HDACs in bortezomib resistant cells. (A) Expression of class II and IV HDACs in myeloma cells (RMPI-8226 and Kas6) was measured by RT-PCR. Expression of individual HDAC in RMPI-8226v10r, Kas6wt, and Kas6v10r was normalized to RPMI-8226wt and plotted in separate figures. (B) HDAC6 protein expression was measured using immunoblotting. Results were quantified from three replicates by measuring band intensity using ImageJ. Quantitative results are displayed graphically next to the immunoblotting image from a representative blot. (C) HDAC7 protein expression was measured using immunoblotting. Results were quantified from three replicates by measuring band intensity using ImageJ. Quantitative results are displayed graphically next to the immunoblotting image from a representative blot. HDACs: Histone deacetylases.