fig5

ABCA1 is associated with the development of acquired chemotherapy resistance and predicts poor ovarian cancer outcome

Figure 5. ABCA1 protein expression is upregulated in ovarian cancer cells with acquired carboplatin resistance. ABCA1 expression in OVCAR-5 (A) and CaOV3 (C) and carboplatin resistant OVCAR-5 CBPR (B) and CaOV3 CBPR (D) by immunocytochemistry using rabbit polyclonal ABCA1 antibody (1/100, NB400-105, Novus Biological). (E) OVCAR-5 cells with Rabbit IgG and (F) CaOV3 cells with Rabbit IgG. (G) Protein extracts from OVCAR-5 (~30 µg) and CaOV3 cell lines (~60 µg) were electrophoresed and immunoblotted with rabbit polyclonal ABCA1 antibody (1/1000, NB400-105, Novus Biological), and β-actin (1/2000, Abcam) was used as a loading control. A major band was detected at ~250 kDa, which is the predicted size for ABCA1. (H) Quantitation of ABCA1 Western blots. Data are from 2-4 independent experiments. Statistical significance was determined using the Student’s t-test, *P < 0.05.

Cancer Drug Resistance
ISSN 2578-532X (Online)

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