fig4

Figure 4. Significant enrichment in Chorismate and reduction in the tryptophan biosynthesis pathway. Volcano plots illustrate significantly altered metabolic pathways and their associated genera between choline and control groups (A and B), and ChoGOS versus choline (C and D). Microbial pathway abundance in the community was profiled using the HUMAnN pipeline, and differential analysis was performed using MaAsLin2 with a general linear model (feature ~ group). Volcano plots were generated in R. Red: P value 2; Blue: P value 2. Black: not significant. Cho: choline; GOS: galactooligosaccharides.