fig2

Figure 2. Effect of Ch on microbiota in WD fed mice: (A) PCoA plots demonstrating there is a shift in microbial composition in WD-fed mice administered cholestyramine (WD+Ch, blue) from that of the WD-fed, cholestyramine-free group (red) and mice administered a normal chow diet (green). ANOSIM R-values for Experiment 1 and Experiment 2 are 0.957 and 0.966, respectively, and each has a P-value < 0.001; right plot shows alpha diversity Shannon index; (B) family level alpha diversity is significantly higher in the WD+Ch group (Mann-Whitney U P-value < 0.001); (C) relative abundances of the families found in each mouse. Ch: Cholestyramine; WD: Western diet; WD+Ch: WD with cholestyramine.