fig2

Figure 2. Differential metabolites calculated by OPLS-DA between (A) MF-A and MLS-A and (B) MF-A and MF-E. The selection criteria of the compounds were those whose VIP value was greater than 2 (A) or 2.5 (B). The principle of sample selection was based on the cluster results in Figure 1B, which were classified into different clusters. Asterisk refers to statistical significance, ^***P < 0.001. MF: Manjing leaves; MLS: mustard leaf stem; VIP: variable important in projection.