fig5

Figure 5. Bidirectional interactions of B. breve I1-B. longum M12 pair. Growth profiles of bifidobacterial strains in monoculture and co-culture. Growth of strains in mono- and co-cultures at 24 and 48 h (OD_620nm at 0 h was the initial inoculation value). The analysis was performed using technically duplicated data (mean ± SD). Two-way ANOVA and Tukey’s tests were performed to determine significant differences between assays (^*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001). B. breve I1: primary degrader, B. longum M12: secondary degrader. ANOVA: Univariate analysis of variance or two-way analysis of variance; LNnT: lacto-N-neotetraose; LNT: lacto-N-tetraose; 2’-FL: 2’-fucosyllactose; 3’-SL: 3’-sialyllactose.