fig3

Figure 3. Bidirectional interactions of B. bifidum JCM 1254-B. longum M12 pair. Growth profiles of bifidobacterial strains in monoculture and co-culture. The growth of the strains in mono- and co-culture was measured at 0, 24, and 48 h. The analysis was performed using technically duplicated data (mean ± SD). Two-way ANOVA and Tukey’s tests were performed to determine significant differences between assays (*P < 0.05, **P < 0.01). B. bifidum JCM 1254: primary degrader, B. longum M12: secondary degrader. LNnT: Lacto-N-neotetraose; LNT: lacto-N-tetraose; 2’-FL: 2’-fucosyllactose; 3’-SL: 3’-sialyllactose.