fig3

Figure 3. Purification of φ13 Mor and characterization of interaction with φ13 CI. (A) Analysis of Mor purification progress on 15% SDS-PAGE, showing a single band for Mor in SEC elution fractions. Each type of fraction loaded is indicated above the lane. Fractions named FT contain the flowthrough from HisTrap affinity chromatography, while numbers listed below gel filtration step indicate the elution volume of each fraction; (B) SEC chromatograms of φ13 Mor. The absorbance at 280 and 260 nm are shown in blue and red, respectively, and the estimated oligomeric state is marked by an arrow; (C) IEF electrophoresis of CI and Mor alone and in complex. The pH standard values are listed on the left and the protein sample composition for each lane is above. CI: Phage repressor; SEC: selected gel filtration; IEF: isoelectric focusing.