fig5

Figure 5. Lesion formation in E. coli Lemo21 (DE3) cells synthesizing MS2-L. Toxin expression was induced at OD₆₀₀ = 0.27 with 1 mM IPTG and samples were taken 60 min after induction and immediately vitrified. Electron microcopy images were acquired with a Titan Krios and a K2 Summit direct detector. Different stages of lesion formation are shown in a proposed sequential order. Data were acquired at a magnification of 81,000x at a defocus of -50 µm and with 30-40 e-/Å total dose (scale bar = 0.05 µM). OM (outer membrane), IM (inner membrane) and PG (peptidoglycan) are indicated by arrows. The fission zone is indicated by brackets. IPTG:Isopropyl-β-D-thiogalactopyranoside.