fig4

Figure 4. Cluster formation of full-length MS2-L derivatives in E. coli Lemo21 (DE3) bacterial cells. Toxin expression was induced at OD₆₀₀ = 0.3 with 1 mM IPTG and samples were taken at defined time points after induction. All images were acquired with an LSM700 confocal microscope with a 63× objective (scale bar = 5 µm). (A) Cells synthesizing MS2-L-GFP 45 min after induction; (B) Cells synthesizing mScarlet-MS2-L 40 min after induction; (C) Propidium iodide staining of cells synthesizing MS2-L-GFP 45 min after induction and of Lemo21 (DE3) control cells. The vast majority of the control cells remain unstained; (D) Co-expression of MS2-L-GFP and mScarlet-MS2-L, 20 and 40 min after induction. GFP: Green fluorescent protein; IPTG: isopropyl-β-D-thiogalactopyranoside.