fig3

Figure 3. Oligomeric assembly of MS2-L derivatives in NDs and in detergent environments. MS2-L derivatives were CF expressed in the presence of either 60 µM DMPG NDs (black traces) or 0.4% Brij78 (red traces), purified and subjected to LILBID analysis. Samples were synthesized in the standard A19 lysate (left panel) or in BW25113ΔDnaJ lysate (right panel). Pictograms illustrate complexes assigned to the respective peak. For reasons of clarity, not each peak has been assigned a pictogram. Laser intensities were adjusted between 10 and 20 mJ to optimize ion yield while preserving high-order complexes. Asterisks mark prominent peaks representing impurities. The individual MS2-L derivatives are indicated. DMPG: 1,2-dimyristoyl-sn-glycero-3-phospho-(1’-rac-glycerol); LILBID: laser-induced liquid bead ion desorption; ND: nanodisc.