fig4

Figure 4. Susceptibility of the single colony isolates from the end time point of the propagation experiment to the three lactococcal phages used in the cocktail: ϕTIFN1 (ϕ1), ϕTIFN5 (ϕ5), ϕTIFN7 (ϕ7) and to the phages present in the supernatant of the blends at the end time point (538 generations). Replicates A-1 and A-2 were used as control - no phage cocktail was added. Replicates B-1 and B-2 were challenged at the onset of the experiment with a cocktail of three lactococcal phages: ϕTIFN1, ϕTIFN5 and ϕTIFN7. The strains 1.01 to 1.50 were isolated from blend A-1, strains 2.01 to 2.50 were isolated from blend A-2, strains 3.01 to 3.50 were isolated from blend B-1 and strains 4.01 to 4.50 were isolated from blend B-2 (for a detailed description see Materials and Methods section).