fig3

Figure 3. Translocation of bacteria (green) and Lpps (red) to bone marrow cells from MIA-induced (A, B) or sham (C) rats. The two fluorescent readings (red: TRITC-labeled Lpps or MAE; green: CFSE labeled bacteria) were merged to detect cells capturing Lpps or MAE (red arrow), bacteria (green arrow) or both bacteria and Lpps (dotted green arrow). TRITC-Labelled Lpps were given at the dose ranging 0.8-1.1 mg/Kg body weight (A, C). Uptake of the single labelled Lpps was detected (red arrow) in APC+ from the left femur of OA rat (A) or in APC(-) compartment from the right tibia of sham rat (C). TRITC-labelled MAE was detected either in APC (+) or APC (-) compartment of femur or tibia (B: APC(-) compartment from the right tibia). Lpps was not detected following administration of the low dose (140 µg/kg) although bacteria were detected in BM APC(+) from right joint (3.5%-6.6 % total APC(+) and the left one [8.8%-12.4 % total APC (+)].