fig5

Figure 5. In vitro assimilation test of β-L-Arap-(1→3)-L-Ara and larch AGP. Bifidobacterium pseudocatenulatum (B. pse) and B. kashiwanohense (B. kas) strains were used for the in vitro assimilation test. A: Gene clusters containing AAfase in Bifidobacterium species used in the growth test (left). Amino acid sequence identity and coverage between B. pseudocatenulatum MCC10289 and other strains using sequences from B. pseudocatenulatum MCC10289 as a query sequence (right table); B: The growth profile of β-L-Arap-(1→3)-L-Ara (left) (n = 1) and larch AGP (right) (n = 3). The absorbance of media of β-L-Arap-(1→3)-L-Ara was monitored at 0, 17, 24, 41, and 48 h, and that of larch AGP was monitored at 0, 18, 24, 42, and 48 h. The absorbance of the growth medium was calculated by subtracting the absorbance of the medium in the absence of bacteria. Error bars indicate standard deviation (n = 3). The black triangle indicates the sample collection point for residual sugar analysis via HPAEC-PAD or TLC; C: HPAEC-PAD analysis of the culture supernatant of β-L-Arap-(1→3)-L-Ara after incubation for 41 h. β-L-Arap-(1→3)-L-Ara was used as a standard.