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Figure 4. Limosilactobacillus reuteri restored splenic dendritic cell and regulatory T cell populations when colonization of the gut microbiome is delayed. (A) Flow cytometry was used to characterize immune cell populations in the spleen of EC, DC, R2lc, and PB-W1 mice. Representative flow cytometry plots and the gating strategy can be found in Supplementary Figure 2. Statistical significance was determined using a one-way ANOVA with the Tukey-Kramer multiple comparisons test. Symbols represent individual samples, and lines represent the mean ± standard deviation; (B) PCA of the proportions of immune cell populations in the spleen. Ellipses represent the 95% confidence interval, symbols represent individual samples, and PERMANOVA was used to assess whether the clustering was significantly different. Data are representative of two independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. See also Supplementary Tables 3 and 6.