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Figure 3. Comparison of immune cell populations in CONV, early colonized (EC), and delayed colonized (DC) mice. (A) Immune cell populations in the spleen of CONV, EC, and DC mice were characterized using flow cytometry; (B) PCA of the proportions of immune cell populations in the spleen; (C) CD11c+ F4/80- dendritic cells in the MLN of CONV, EC, and DC mice were assessed using flow cytometry. Representative flow cytometry plots and the gating strategy can be found in Supplementary Figure 2. For (A) and (C), statistical significance was determined using one-way ANOVA followed by the Tukey-Kramer multiple comparisons test. Symbols represent individual samples and lines represent the mean ± standard deviation. For (B), ellipses represent the 95% confidence interval, symbols represent individual samples, and PERMANOVAs were used to assess whether the clustering was significantly different. Data are representative of two independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001. See also Supplementary Figures 3 and 4 and Supplementary Tables 2 and 5.