fig4

Figure 4. Accuracy of 16S metagenome-DRIP for estimating the abundance of a minority species. (A) The relative abundances (%) of F. prausnitzii estimated from the results of the conventional 16S metagenome (white bars) and 16S metagenome-DRIP (black bars) analyses. (B and C) The relative abundances (%) of F. prausnitzii determined by qPCR were compared with those deduced from the data of 16S metagenome-DRIP (B) or the conventional 16S-metagenome (C) analysis. Pearson’s correlation coefficient analysis was used to evaluate the data. Subject IDs are indicated (see Table 2). DRIP: Deeper Resolution using an Inhibitory Primer; qPCR: quantitative PCR.