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From: Mitochondria-targeting amphiphilic polymers enable idebenone to promote neuronal regeneration following spinal cord injury

Figure 2. Inhibits RSL3-induced ferroptosis in HT22 cells. (A) Distribution of P@I and SP@I after 2, 6, and 10 h co-incubation with HT22 cells. The scale bar is 10 μm; (B) Half-inhibitory concentration of RSL3-induced HT22 cell death; (C) Cytotoxic effects of P@I and SP@I on HT22 cells after 24 h of co-incubation; (D) The optimal concentration of SP@I for inhibiting RSL3-induced ferroptosis in HT22 cells; (E) Protective effect of 1 μM P@I and SP@I against RSL3-induced ferroptosis in HT22 cells. SP@I versus P@I, P < 0.001; SP@I versus RSL3, P < 0.001; P@I versus RSL3, P < 0.001; (F-G) Analysis and quantification of Flow cytometry of mitochondrial superoxide. SP@I versus P@I, P < 0.001; SP@I vs. RSL3, P < 0.001; P@I versus RSL3, P < 0.001. Data are expressed as the mean ± SD (n = 3). SD: Standard deviation.