fig4

Figure 4. POLG-dependent changes in mt-tRNAs are negatively correlated with amino acid amyloidogenic potential. Log2FC values for significantly altered mt-tRNAs in the POLG mutant (X-axis) were plotted against a series of amino acid characteristic scores (Y-axis) used for predicting amino acid contributions to protein aggregation and beta-amyloid formation (A-D). Linear regression analysis confirms significant negative correlation with FoldAM triple hybrid and regular scoring criteria (A and B). There was a positive correlation with the AA's sum contact potential value (C) and side chain orientation scores (D). These scores can be indicative of the AA's propensity to mediate specific protein:protein interactions. As another validation, group analysis (t-test) confirmed lower FoldAM triple hybrid score for mt-tRNAs that showed significantly increased vs. decreased expression in the POLG mutant (E).