fig1

Figure 1. Engineering pCMV-MIR-214 and generation of iEV-214. (A) Sequence of pre-miR-214 minigene that was cloned into pCMV-MIR to generate pCMV-MIR-214; (B) Restriction mapping of pCMV-MIR (empty) and pCMV-MIR-214. Plasmids were double digested with SgfI and XhoI and fragments were resolved via electrophoresis; (C) Schematic representation depicting the generation of iEV-214; (D) Quantification of iEVs purified from the conditioned media of J558L cells that were untransfected (-) or transfected with pCMV-MIR-scr or pCMV-MIR-214. iEV protein content was quantified using the BCA assay.