fig6

Figure 6. Microtubule-based bidirectional DCV transport. The anterograde axonal transport of DCVs on microtubules is mediated mainly by kinesin-3 and some by other kinesins. Rab2 is required for bidirectional DCV transport in the axon. The Arf-like GTPase, Arl8, is an adaptor for kinesin-3-mediated DCV movement. CPE cytoplasmic tail on DCVs recruits dynactin and KIF1A (kinesin-3) and KIF3A (kinesin-2). KIF1A and KIF3A mediate the anterograde transport of these vesicles on microtubules. Cytoplasmic dynein, a minus-end directed motor, binds dynactin and mediates the return of DCVs from the neurite terminus back to the cell body under non-stimulated conditions for vesicle homeostasis. Dynactin, a microtubule anchor protein complex for cytoplasmic dynein and kinesins, mediates the bidirectional movement of DCVs. CPE: carboxypeptidase E; DCV: dense core vesicles.