fig6

Figure 6. HepG2-derived CD147⁺ sEVs promote endothelial cell angiogenesis in vivo. (A) HUVECs treated with PBS, HepG2-WT-sEVs, HepG2-CD147(KD)-sEVs, and MK-2206 together with HepG2-WT-sEVs were injected subcutaneously into female BALB/c nude mice. Mice were then euthanized and photographed 14 days after injection; (B) Matrigel plugs were excised and photographed 14 days after subcutaneous injection; (C) Volume of matrix plugs in each group from (B); (D) Matrigel plugs were subjected to immunohistochemistry to examine the expression of CD31 in endothelial cells. Original magnification of image: × 100; (E) Quantification of the number of MVD in each group. MVD: microvessel density; ns: no significance; *: P < 0.05; **: P < 0.01; EVs: extracellular vesicles; HUVECs: human umbilical vein endothelial cells; PBS: phosphate‐buffered saline.