REFERENCES
1. Théry C, Witwer KW, Aikawa E, et al. Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines. J Extracell Vesicles 2018;7:1535750.
2. Welsh JA, Van Der Pol E, Arkesteijn GJA, et al. MIFlowCyt-EV: a framework for standardized reporting of extracellular vesicle flow cytometry experiments. J Extracell Vesicles 2020;9:1713526.
3. Spidlen J, Breuer K, Brinkman R. Preparing a Minimum Information About a Flow Cytometry Experiment (MIFlowCyt) compliant manuscript using the International Society for Advancement of Cytometry (ISAC) FCS file repository (FlowRepository.org). Curr Protoc Cytom 2012;Chapter 10:Unit 10.18.
4. Lee JA, Spidlen J, Boyce K, et al. International Society for Advancement of Cytometry Data Standards Task Force. MIFlowCyt: the minimum information about a Flow Cytometry Experiment. Cytometry A 2008;73:926-30.
5. Welsh JA, Tang VA, van der Pol E, Görgens A. MIFlowCyt-EV: the next chapter in the reporting and reliability of single extracellular vesicle flow cytometry experiments. Cytometry A 2021;99:365-8.
6. der Pol E, Welsh JA, Nieuwland R. Minimum information to report about a flow cytometry experiment on extracellular vesicles: communication from the ISTH SSC subcommittee on vascular biology. J Thromb Haemost 2022;20:245-51.
7. Welsh JA, Jones JC, Tang VA. Fluorescence and light scatter calibration allow comparisons of small particle data in standard units across different flow cytometry platforms and detector settings. Cytometry A 2020;97:592-601.
8. van der Pol E, de Rond L, Coumans FAW, et al. Absolute sizing and label-free identification of extracellular vesicles by flow cytometry. Nanomedicine 2018;14:801-10.
9. Tian Y, Gong M, Hu Y, et al. Quality and efficiency assessment of six extracellular vesicle isolation methods by nano-flow cytometry. J Extracell Vesicles 2020;9:1697028.
10. Crooks ET, Almanza F, D'Addabbo A, et al. Engineering well-expressed, V2-immunofocusing HIV-1 envelope glycoprotein membrane trimers for use in heterologous prime-boost vaccine regimens. PLoS Pathog 2021;17:e1009807.
11. Oh CK, Dolatabadi N, Cieplak P, et al. S-Nitrosylation of p62 inhibits autophagic flux to promote α-synuclein secretion and spread in parkinson’s disease and lewy body dementia. J Neurosci 2022;42:3011-24.
12. Sandau US, Duggan E, Shi X, et al. Methamphetamine use alters human plasma extracellular vesicles and their microRNA cargo: an exploratory study. J Extracell Vesicles 2020;10:e12028.
13. Shpigelman J, Lao FS, Yao S, et al. Generation and application of a reporter cell line for the quantitative screen of extracellular vesicle release. Front Pharmacol 2021;12:668609.
14. Yan W, Cao M, Ruan X, et al. Cancer-cell-secreted miR-122 suppresses O-GlcNAcylation to promote skeletal muscle proteolysis. Nat Cell Biol 2022;24:793-804.
15. Nguyen DC, Keller RA, Jett JH, Martin JC. Detection of single molecules of phycoerythrin in hydrodynamically focused flows by laser-induced fluorescence. Anal Chem 1987;59:2158-61.
16. Soper SA, Shera EB, Martin JC, et al. Single-molecule detection of Rhodamine 6G in ethanolic solutions using continuous wave laser excitation. Anal Chem 1991;63:432-7.
17. Zhu S, Ma L, Wang S, et al. Light-scattering detection below the level of single fluorescent molecules for high-resolution characterization of functional nanoparticles. ACS Nano 2014;8:10998-1006.
18. Gaigalas AK, Wang L, Schwartz A, Marti GE, Vogt RF Jr. Quantitating fluorescence intensity from fluorophore: assignment of MESF values. J Res Natl Inst Stand Technol 2005;110:101-14.
19. Schwartz A, Wang L, Early E, et al. Quantitating fluorescence intensity from fluorophore: the definition of MESF assignment. J Res Natl Inst Stand Technol 2002;107:83-91.
20. Wang L, Hoffman RA. Standardization, calibration, and control in flow cytometry. Curr Protoc Cytom 2017;79:1.3.1-1.3.27.
21. Hoffman RA. Standardization, calibration, and control in flow cytometry. Curr Protoc Cytom 2005;Chapter 1:Unit 1.3.
22. der Pol E, van Gemert MJ, Sturk A, Nieuwland R, van Leeuwen TG. Single vs. swarm detection of microparticles and exosomes by flow cytometry. J Thromb Haemost 2012;10:919-30.
23. Rond L, Coumans FAW, Welsh JA, Nieuwland R, van Leeuwen TG, van der Pol E. Quantification of light scattering detection efficiency and background in flow cytometry. Cytometry A 2021;99:671-9.
24. Keller RA, Ambrose WP, Goodwin PM, Jett JH, Martin JC, Wu M. Single-molecule fluorescence analysis in solution. Appl Spectrosc 1996;50:12A-32A.
