fig4
Figure 4. Analysis of the mitochondrial network skeletons in NPCs using MiNA. (A) Confocal images of NPCs stained with DAPI (blue) and TOM20 (green) for tracking the mitochondrial membrane. MiNA plug-in on ImageJ was used to skeletonize the mitochondria. Box and whisker plots quantify the (B) mitochondrial footprint, (C) total mitochondrial networks, (D) mean branch length, and (E) total branch length. Box plots show the mean (horizontal line), first to third quartile (box), and the extreme values within the 95% confidence interval of the interquartile range (whiskers). Scale bar for representative images is 20 µm. *P < 0.05, **P < 0.01, ***P < 0.001, and
