fig5

Figure 5. Role of human primary microglia-derived exosomes on neuronal miniature excitatory postsynaptic currents (mEPSC). (A) Representative traces of whole-cell voltage-clamp recording showing mEPSC, (B) mean mEPSC frequencies (Hz), and (C) amplitude (pA) in primary rat hippocampal neurons (DIV 18-22) exposed to MDEVs from control, Tat, NLRP3 silenced and NLRP3 silenced- Tat treated HPM cells. Data are presented as mean ± SEM. *P < 0.05 vs. siControl MDEV, ^#P < 0.05 vs. Tat MDEV. One-way ANOVA, followed by the Bonferroni post hoc tests, was used for statistical analysis. Scramb: Scrambled siRNA (small interfering RNA); MDEV: microglia derived exosomes; NLRP3: NOD-, LRR- and pyrin domain-containing protein 3; PSD95: postsynaptic density protein 95; vGLUT1: vesicular glutamate transporters; GAD65: glutamic acid decarboxylase; siRNA: small interfering RNA; Tat: trans-activator of transcription.