fig6
Figure 6. Free flow electrophoresis (FEE) is reproducible, allowing preparation of extracellular vesicles (EVs) from MSC-CMs. Five MSC-CMs were fractioned on the same day by FFE applying the protocol indicated in Figure 4. To evaluate the reproducibility of the EV preparation, obtained Fractions 7-10 were analyzed: (A) following anti-CD9 labeling by imaging flow cytometry; (B) by nanoparticle tracking analysis; and (C) following chloroform-methanol precipitation by anti-CD9 Western blot (exposure time 270 s).
