REFERENCES
1. Lancet. Controlling asthma. Lancet 2014;383:1521.
2. Zar HJ, Ferkol TW. The global burden of respiratory disease-impact on child health. Pediatr Pulmonol 2014;49:430-4.
3. Ramratnam SK, Bacharier LB, Guilbert TW. Severe Asthma in Children. J Allergy Clin Immunol Pract 2017;5:889-98.
4. Dharmage SC, Perret JL, Custovic A. Epidemiology of Asthma in Children and Adults. Front Pediatr 2019;7:246.
5. Wenzel SE. Asthma phenotypes: the evolution from clinical to molecular approaches. Nat Med 2012;18:716-25.
6. Wu G, Yang G, Zhang R, et al. Altered microRNA Expression Profiles of Extracellular Vesicles in Nasal Mucus From Patients With Allergic Rhinitis. Allergy Asthma Immunol Res 2015;7:449-57.
7. Levänen B, Bhakta NR, Torregrosa Paredes P, et al. Altered microRNA profiles in bronchoalveolar lavage fluid exosomes in asthmatic patients. J Allergy Clin Immunol 2013;131:894-903.
8. Mortaz E, Alipoor SD, Varahram M, et al. Exosomes in Severe Asthma: Update in Their Roles and Potential in Therapy. Biomed Res Int 2018;2018:2862187.
9. Sastre B, Cañas JA, Rodrigo-Muñoz JM, Del Pozo V. Novel Modulators of Asthma and Allergy: Exosomes and MicroRNAs. Front Immunol 2017;8:826.
10. Palanisamy V, Sharma S, Deshpande A, Zhou H, Gimzewski J, Wong DT. Nanostructural and transcriptomic analyses of human saliva derived exosomes. PLoS One 2010;5:e8577.
11. Sharma S, Rasool HI, Palanisamy V, et al. Structural-mechanical characterization of nanoparticle exosomes in human saliva, using correlative AFM, FESEM, and force spectroscopy. ACS Nano 2010;4:1921-6.
12. Lässer C, Alikhani VS, Ekström K, et al. Human saliva, plasma and breast milk exosomes contain RNA: uptake by macrophages. J Transl Med 2011;9:9.
13. Wong DT. Salivary Diagnostics: Amazing as it might seem, doctors can detect and monitor diseases using molecules found in a sample of spit. Am Sci 2008;96:37-43.
14. Kaczor-Urbanowicz KE, Martin Carreras-Presas C, Aro K, Tu M, Garcia-Godoy F, Wong DT. Saliva diagnostics - Current views and directions. Exp Biol Med (Maywood) 2017;242:459-72.
15. Hyyppä T. Studies on immunologic and inflammatory factors in the saliva and gingiva in patients with asthma. Journal of Clinical Periodontology 1981;8:500-7.
16. Siegler DI, Citron KM. Serum and parotid salivary IgA in chronic bronchitis and asthma. Thorax 1974;29:313-6.
19. Butz A, Bellin MH, Bollinger ME, et al. Salivary cotinine measurement for all children with persistent asthma: spit matters. Ann Allergy Asthma Immunol 2016;116:463-5.
20. Negretti F, Casetta P. Remarkable increases of salivary IgE levels in allergic syndromes. Int Arch Allergy Appl Immunol 1990;92:103-4.
21. Little FF, Delgado DM, Wexler PJ, et al. Salivary inflammatory mediator profiling and correlation to clinical disease markers in asthma. PLoS One 2014;9:e84449.
22. Phipatanakul W, Koutrakis P, Coull BA, et al. The School Inner-City Asthma Intervention Study: Design, rationale, methods, and lessons learned. Contemp Clin Trials 2017;60:14-23.
23. Zlotogorski-Hurvitz A, Dayan D, Chaushu G, et al. Human saliva-derived exosomes: comparing methods of isolation. J Histochem Cytochem 2015;63:181-9.
24. Comfort N, Cai K, Bloomquist TR, Strait MD, Ferrante AW, & Baccarelli AA. Novel nanoparticle tracking analysis technology and method for extracellular vesicle quantification and size determination. J Vis Exp. Forthcoming 2021.
25. Gardiner C, Ferreira YJ, Dragovic RA, Redman CW, Sargent IL. Extracellular vesicle sizing and enumeration by nanoparticle tracking analysis. J Extracell Vesicles 2013;2:19671.
26. Core TeamR. R Foundation for Statistical Computing. (2018). Available from: https://www.r-project.org/index.html [Last accessed on 18 Mar 2021].
27. Harris VM. Protein Detection by Simple Western™ Analysis. In: Kurien BT, Scofield RH, editors. Western Blotting. New York: Springer; 2015. pp. 465-8.
28. Chen JQ, Heldman MR, Herrmann MA, et al. Absolute quantitation of endogenous proteins with precision and accuracy using a capillary Western system. Anal Biochem 2013;442:97-103.
29. Lötvall J, Hill AF, Hochberg F, et al. Minimal experimental requirements for definition of extracellular vesicles and their functions: a position statement from the International Society for Extracellular Vesicles. J Extracell Vesicles 2014;3:26913.
30. Aqrawi LA, Galtung HK, Guerreiro EM, et al. Proteomic and histopathological characterisation of sicca subjects and primary Sjögren's syndrome patients reveals promising tear, saliva and extracellular vesicle disease biomarkers. Arthritis Res Ther 2019;21:181.
31. Nonaka T, Wong DT. Saliva-Exosomics in Cancer: Molecular Characterization of Cancer-Derived Exosomes in Saliva. Peptidomics of Cancer-Derived Enzyme Products. Elsevier; 2017. pp. 125-51.
32. Wexler PJ, Siqueira WL, Helmerhorst EJ, Blicharz TM, Hayman RB, et al. Saliva Diagnostics in Asthma. Proceedings of the American Thoracic Society 2009:6.
33. Colombo M, Raposo G, Théry C. Biogenesis, secretion, and intercellular interactions of exosomes and other extracellular vesicles. Annu Rev Cell Dev Biol 2014;30:255-89.
34. Szatanek R, Baj-Krzyworzeka M, Zimoch J, Lekka M, Siedlar M, Baran J. The Methods of Choice for Extracellular Vesicles (EVs) Characterization. Int J Mol Sci 2017;18:1153.
35. Niel G, D’Angelo G, & Raposo G. Shedding light on the cell biology of extracellular vesicles. Nat Rev Mol Cell Biol 2018;19:213-28.
36. Théry C, Witwer KW, Aikawa E, et al. Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines. J Extracell Vesicles 2018;7:1535750.
37. van der Pol E, Coumans FA, Grootemaat AE, et al. Particle size distribution of exosomes and microvesicles determined by transmission electron microscopy, flow cytometry, nanoparticle tracking analysis, and resistive pulse sensing. J Thromb Haemost 2014;12:1182-92.
38. der Pol E, Hoekstra AG, Sturk A, Otto C, van Leeuwen TG, Nieuwland R. Optical and non-optical methods for detection and characterization of microparticles and exosomes. J Thromb Haemost 2010;8:2596-607.
39. Bachurski D, Schuldner M, Nguyen PH, et al. Extracellular vesicle measurements with nanoparticle tracking analysis - An accuracy and repeatability comparison between NanoSight NS300 and ZetaView. J Extracell Vesicles 2019;8:1596016.
40. Vogel R, Savage J, Muzard J, et al. Measuring particle concentration of multimodal synthetic reference materials and extracellular vesicles with orthogonal techniques: Who is up to the challenge? J Extracell Vesicles 2021;10:e12052.
41. Skliar M, Chernyshev VS, Belnap DM, et al. Membrane proteins significantly restrict exosome mobility. Biochem Biophys Res Commun 2018;501:1055-9.
42. Goetzl EJ, Yaffe K, Peltz CB, et al. Traumatic brain injury increases plasma astrocyte-derived exosome levels of neurotoxic complement proteins. FASEB J 2020;34:3359-66.
43. Abner EL, Elahi FM, Jicha GA, et al. Endothelial-derived plasma exosome proteins in Alzheimer's disease angiopathy. FASEB J 2020;34:5967-74.
44. Daaboul GG, Gagni P, Benussi L, et al. Digital Detection of Exosomes by Interferometric Imaging. Sci Rep 2016;6:37246.
45. Helwa I, Cai J, Drewry MD, et al. A Comparative Study of Serum Exosome Isolation Using Differential Ultracentrifugation and Three Commercial Reagents. PLoS One 2017;12:e0170628.
46. Erdbrügger U, Lannigan J. Analytical challenges of extracellular vesicle detection: A comparison of different techniques. Cytometry A 2016;89:123-34.
47. Azmeh R, Greydanus DE, Agana MG, et al. Update in Pediatric Asthma: Selected Issues. Dis Mon 2020;66:100886.
48. Gemoll T, Rozanova S, Röder C, et al. Protein Profiling of Serum Extracellular Vesicles Reveals Qualitative and Quantitative Differences After Differential Ultracentrifugation and ExoQuickTM Isolation. J Clin Med 2020;9:1429.
49. Jeyaram A, Jay SM. Preservation and Storage Stability of Extracellular Vesicles for Therapeutic Applications. AAPS J 2017;20:1.
50. Yuana Y, Böing AN, Grootemaat AE, et al. Handling and storage of human body fluids for analysis of extracellular vesicles. J Extracell Vesicles 2015;4:29260.