fig9

Figure 9. (A) Graphical synthetic route of CTp and mCTpBD, respectively, and enrichment procedure of glycopeptides by mCTpBD; (B)TEM images of mCTpBD; MALDI-TOF MS of HRP tryptic digest (100 fmol/μL) (C) before the enrichment, and (D) after the enrichment by mCTpBD. The peaks of glycopeptides are marked with a red asterisk (*); (E) The synthetic route of Fe₃O₄@TpBD@Au@GSH; (F) SEM of Fe₃O₄@TpBD@Au@GSH; MALDI-TOF MS of the mixture of HRP tryptic digests and BSA proteins at various molar ratios after enrichment by Fe₃O₄@TpBD@Au@GSH (G) 1:1,000, (H) 1:2,000. (A-D) are reprinted with permission from Ref.^[116], Copyright © 2020 by American Chemical Society. (E-H) are reproduced with permission from Ref.^[128], Copyright © 2022 by Elsevier. CTp: Carboxyl-modified 1,3,5-triformylphloroglucinol; mCTpBD: magnetic carboxyl-modified 1,3,5-triformylphloroglucinol-benzidine covalent organic framework; TEM: transmission electron microscopy; MALDI-TOF MS: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry; HRP: horseradish peroxidase; Tp: 2,4,6-trihydroxybenzene-1,3,5-tricarbaldehyde; BD: benzidine; GSH: glutathione; SEM: scanning electron microscope; BSA: bovine serum albumin.